Estrogen Regulates the Tumour Suppressor MiRNA-30c and Its Target Gene, MTA-1, in Endometrial Cancer
Figure 2
Variation of miR-30c expression modulates the expression of its target gene, MTA-1, in EC cells.
(A). The transfection efficiency of Ishikawa cells was evaluated by qRT-PCR at 48 h after transfection of miR-30c-mimics and miR-30c-inhibitor, shown as fold-changes relative to their scrambled controls. (B). MTA-1 protein expression was assessed by western blot analysis at 48 h after transfection with miR-30c-mimics, miR-30c-inhibitor and their scrambled controls. (C). Cotransfection with miR-30c inhibitor, siR-MTA-1 and their scrambled controls was performed, and MTA-1 protein expression was assessed by western blot analysis. (D and E). SiR-MTA-1 reduces MTA-1 expression at the mRNA (D) and protein (E) levels at 48 h after transfection. (F). QRT-PCR analysis shows an increase in miR-30c expression after MTA-1 expression was inhibited. Each bar represents the mean values ± SD from three independent experiments. (*P<0.05, **P<0.01).