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Altering the Architecture of Tissue Engineered Hypertrophic Cartilaginous Grafts Facilitates Vascularisation and Accelerates Mineralisation

Figure 7

Histology and immunohistochemistry of the channels within channelled constructs pre-implantation and 4 weeks post-implantation.

Channels were examined to determine the pathway through which bone formation occurs. (A,E) Alcian blue staining. (B,F) Collagen I staining. (C,G) Collagen II staining. (D,H) Collagen X staining. (A–D) Pre-implantation. (E–H) 4 weeks post-implantation. Scale bars are 50 µm.

Figure 7

doi: https://doi.org/10.1371/journal.pone.0090716.g007