Widespread Nanoparticle-Assay Interference: Implications for Nanotoxicity Testing
Figure 4
Assessment of nanoparticle interference with the assays components, with addition of analyte.
(A) BCA protein assay with 250 µg/mL BSA addition; (B) Bradford protein assay with 40 µg/mL BSA addition; (C) LDH assay with 12.5×106 lysed cells; (D) Catalase assay with 250 U/mL catalase addition (excitation 531 nm, emission 595 nm); (E) Catalase assay with 250 U/mL catalase addition (absorbance 560 nm). * indicates significantly different than control (p<0.05, ANOVA followed by Dunnett's post-hoc comparison).