Imaging Neuroinflammation In Vivo in a Neuropathic Pain Rat Model with Near-Infrared Fluorescence and 19F Magnetic Resonance
Figure 2
Near Infrared imaging (NIR) of live rats merged with white light images of their body, 2 days post intravenous injection of labeled emulsion on day 11 post surgery.
All of these images were acquired on the LiCOR Pearl Live animal imager and processed in the same experiment with linked look-up tables. The control (A, B, C) and sham (D, E, F) exhibit only auto fluorescence over the thoracolumbar region, which is evident prior to injection (A, D, G). The inset in F exhibits a dorsal view of the wound, which lacks any fluorescent signal. The CCI affected right leg (I) exhibits a clear band of fluorescent signal aligned with the position of the sciatic nerve (arrow), which runs parallel to the femur. No signal is evident on the CCI animal’s unaffected left leg (H). Note – supplemental data (Figure S3) of a comparable experiment reveals only autofluoresence in sham and naïve controls, while only the CCI exhibits extensive signal along the length of the thigh, parallel to the position of the right sciatic nerve. Bar = 1 cm.