IL-10-Producing B Cells Are Induced Early in HIV-1 Infection and Suppress HIV-1-Specific T Cell Responses
Figure 4
TIM-1+ B cells were enriched with HIV-1 trimeric Env-specific B cells.
PBMCs were incubated with a FLAG-tagged HIV-1 YU2 gp140 trimer (see Materials and Methods) and then stained with anti-FLAG mAb, CD3, CD19, IgD, and TIM-1 mAbs. (A) Representative flow cytometry dot plot showing gp140 and TIM-1 co-staining of IgD− B cells from an HIV-1 viremic individual. Staining of the same HIV-1 viremic individual without gp140 trimer (No gp140) and staining of an HIV-1 uninfected subject (HIV-1 uninfected) were used as gating controls for gp140 gating. (B) Summary of gp140 staining on TIM-1+ IgD− and TIM-1− IgD− B cells from five HIV-1 viremic individuals. **: P<0.01. (Wilcoxon rank-sum test).