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Effect of Bodily Fluids from Honey Bee (Apis mellifera) Larvae on Growth and Genome-Wide Transcriptional Response of the Causal Agent of American Foulbrood Disease (Paenibacillus larvae)

Figure 1

Effect of honey bee larval bodily fluid on Paenibacillus larvae growth.

Effect of different concentrations of honey bee larval bodily fluid on the in vitro growth of P. larvae bacterial cells, expressed as the optical density measured at a wavelength of 590 nm (OD590) in function of time (hours). Growth alterations were determined for six bodily fluid concentrations, expressed as the fold dilution in BHIT broth cultures: 10× dilution (♦), 25× dilution (▪), 50× dilution (▴), 100× dilution (⋄), 250× dilution (□), 500× dilution (▵), control (○). Each point in the graph displays the mean of three independent replicates, with the error bars being the standard deviations. Time 0 represents the time of spiking. Trend lines are calculated with DMfit. No OD measurements were performed during lag phase.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0089175.g001