CCR5 Conformations Are Dynamic and Modulated by Localization, Trafficking and G Protein Association
Figure 3
Surface and Total CCR5 conformation expression within U87.CD4.CCR5-GFP cells.
U87.CD4.CCR5-GFP cells were incubated with 2D7, 45531, PA11 or MC-5 for surface (grey bars) or total labeling (black bars). CCR5 MAb labeling was measured using an IDL program and represented as a percentage of total CCR5. Graphs display averages of 4 independent experiments (N = 30 cells per experimental condition). Statistical analysis was performed using an unpaired t test. Error bars represent Standard Error of the Mean (SEM), ****p<.0001, **p<.01, *p<.05