Stromal Cells from Human Decidua Exert a Strong Inhibitory Effect on NK Cell Function and Dendritic Cell Differentiation
Figure 5
DSC express IDO and produce PGE2.
(A) Analysis of IDO mRNA expression in DSCs isolated from different patients (pt). (B) IDO mRNA expression was analyzed in IDO− DSCs cultured alone (first line) or with IL15-activated NK cells in cell-to-cell contact or Tw conditions or after ON incubation with supernatant (spt) of IL15-activated NK cells or 100 UI of IFN-γ. RT–PCR was performed with primers specific for IDO and for β-actin as positive control. PCR products were run on a 0.8% agarose gel and visualized by ethidium bromide staining. (C) PGE2 production was analyzed in supernatants by DSCs culture alone (black bar) or with IL15-activated NK cells. (D) Analysis of Jagged-1 mRNA expression in DSCs isolated from different patients.