Loss of Polycystin-1 Inhibits Bicc1 Expression during Mouse Development
Figure 2
Expressional profiles of Bicc1 protein during embryogenesis and kidney development.
By immunohistochemistry (IHC) staining (n≥3), (A) positive labeling is observed in the neural tube (arrow) at E8.5, * indicates the perineural tube mesenchymal tissue; (B) cardiomyocytes in the myocardial wall (arrows) at E9.5, * indicates the pericardio-peritoneal canal; (C) the primordial gut (arrow), * indicates the peritoneal cavity at E10.5; (D) immature hepatocytes (arrow) at E10.5; (E) epithelia of the main bronchi (arrows), + indicates the pericardio-peritoneal canal and * indicates the lung bud tissue; (F) the aortic wall (arrow), * indicates the cardinal vein; and (G) early ureteric bud (lower arrow)/mesonephric duct (upper arrow) at E11.5; (H) the renal comma-shaped body (arrows), (I) epithelia of the pancreatic primordium (arrows), * indicates hepatic tissue and (J) the posterior root ganglions at E12.5, * indicate vertebral bodies. Bicc1 appeared in (K) the adrenal cortex (lower arrow) and cortex of the kidney (upper arrow), * indicates the liver at E16.5; (L) olfactory epithelia (arrows) at E16.5, + indicates the primordial turbinate bone and * indicate the nasal cavities. (M) IHC with mBicc1p antiserum showed positive labeling (arrow) in the newborn mouse kidney. (N) In the 1-month-old kidney, positive staining (arrow) was observed in the juxta-medullary region of the kidney. (O) In the 3-month-old kidney, clear-cut Bicc1 expression (arrow) was seen a similar region of the kidney, but some positive labeling also appeared over the cortical region. (P) Besides of the juxta-medullary and cortical regions, positive labeling also extended to the medullary regions in the 6-month-old kidney. All high-power positive images were from the arrow-pointed regions and placed on the left-lower corners of M–O. (M'–P') showed IHC labeling without counterstaining for the corresponding regions of M–O, respectively. Bars: 10 µm in D; 20 µm in A, C, G–H; 25 µm in E and I; 50 µm in B, F, J–L; 100 µm in M; 150 µm in N–O; 300 µm in P.