The Ctf18RFC Clamp Loader Is Essential for Telomere Stability in Telomerase-Negative and mre11 Mutant Alleles
Figure 2
Senescence of telomerase-negative strains in the presence and absence of Rad52.
[A]: Cells were subcultured for eleven rounds (198 generations post germination) of subculturing for the indicated strains as described above. Colonies from freshly dissected spores were incubated on YPD plates for 36 hours at 30°C, and 1×105 cells were inoculated into 5 ml of YPD at 30°C for 18 hours [54 generations after dissection], and cells counted using a hemocytometer. Each subsequent 18-hour round of grown [hours 72–198] was initiated by inoculating 1×105 cells into fresh YPD. [B]: Telomere lengths of the indicated strains in [2A] from hours 54, 72 and 90 hours after dissection were determined by Southern analysis of XhoI digested DNA using poly GT as a probe.