The NRF2-KEAP1 Pathway Is an Early Responsive Gene Network in Arsenic Exposed Lymphoblastoid Cells
Figure 8
Response of Nrf2-regulated genes to iAs exposure.
Evaluation of mRNA levels of the indicated Nrf2-target genes by RT-qPCR in CL-1 cell line after exposure to 5 µM of iAs in a time-response curve. A) HMOX1 and NQO1 expression after dose-response exposure at 12 hrs, B) HMOX1 and NQO1 time response at 5 µM of iAs. Data values are means ± SD and were normalized using GADPH as an endogenous gene and fold changes were calculated using the ΔΔCt method. Three independent cell cultures were used for each assay data point. **p<0.001, *p<0.01.