NADPH Oxidase 4 Deficiency Reduces Aquaporin-2 mRNA Expression in Cultured Renal Collecting Duct Principal Cells via Increased PDE3 and PDE4 Activity
Figure 1
Increased AQP2 expression by a vasopressin analogue is decreased by NOX4 depletion in mpkCCDcl4 cells.
mRNA expression levels of NOX4, Na,K-ATPase α subunit (NaKα), Na,K-ATPase β subunit (NaKβ) and AQP2 (A) and HNF3 and UT-A1 (B) were compared by Q-PCR in mpkCCDcl4 cells transfected with either scramble siRNA (scr) or siRNA against NOX4 (siNOX4) and challenged or not (Ctl) 24 h with either desmopressin, a V2R-selective analogue of vasopressin (VP, 10−9 M), or NaCl-hypertonic medium (500 mOsmol/kg). Data is represented as fold induction over non-stimulated cells transfected with scramble siRNA and is expressed as the mean ± SEM of six independent experiments. *P≤0.05; NS: no significant differences.