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Expression and Purification of Chaperone-Active Recombinant Clusterin

Figure 1

Images of Coomassie blue stained SDS-PAGE gels analysing b-rCLU.

(A) Lane 1: Molecular weight markers with size indicated in kDa. Lane 2: Sample of b-rCLU after SEC purification in solubilisation buffer containing 8 M urea and 5 mM DTT. (B) Lane 1: Molecular weight markers with size indicated in kDa. Lane 2: Sample of b-rCLU after step-wise dialysis into PBS/Az.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0086989.g001