STAT3 but Not STAT1 Is Required for Astrocyte Differentiation
Figure 2
Overexpression of STAT3 in the chick spinal cord induces differentiation of astrocytes.
(A–C) Expression of glial markers Hes5 and GLAST in STAT3-overexpressing chick spinal cords at D6, which is not changed. (D–L) Expression of GFP, NFIA, H5 and GFAP in D15 chick spinal cords electroporated with GFP, STAT3 or STAT3CA. Insets are magnified images. GFP+ processes express H5 and GFAP in STAT3 or STAT3CA-electroporated groups (arrows, H, I, K, L), unlike the ones in control (arrowheads, D, G, J). Dotted lines mark the boundary of neural tube. (M, N) Quantification of NFIA and H5-expressing cells in the marginal zone. Error bars represents s.e.m. *p<0.05, ***p<0.001 vs. GFP; unpaired-Student’s t-test. Scale bars: in C, 100 µm for A–C; in L, 100 µm for D–L.