Collagen XVI Induces Expression of MMP9 via Modulation of AP-1 Transcription Factors and Facilitates Invasion of Oral Squamous Cell Carcinoma
Figure 5
Collagen XVI overexpression leads to increased invasion of OSCC cells.
(A) Quantitative PCR of MMP9 gene expression of COLXVI and mock control cells after AP-1 inhibition with Tanshinone IIA (TIIA; c = 100 ng/mL). The expression of MMP9 decreased in the COLXVI cell clones after AP-1 inhibition with Tanshinone IIA. (n = 3) (B) 3D micromass pellets of COLXVI cells compared to mock control cells 24 h after placement. COLXVI cells (high and low expressing) show a significantly wider invasion zone (orange line) compared to mock control cells (p<0.01; n = 10). (C) 3D micromass pellets of mock control cells after 24 h of incubation with 500 ng/mL recombinant collagen XVI. Incubation with recombinant collagen XVI resulted in a significantly increased spreading of the invasion zone (orange line) compared to the control (p<0.001; n = 10).