Starvation Increases Insulin Sensitivity and Reduces Juvenile Hormone Synthesis in Mosquitoes
Figure 2
Starvation decreases JH synthesis and JHAMT transcript levels.
CA-CC complexes were dissected from 4 days old females after 3 days feeding water (0%) or 20% sucrose (20%). A) JH synthesis: groups of 3 glands were incubated for 4 h; JH synthesis was evaluated by HPLC-FD and it is expressed as fmol per CA per hour. Bars represent the means ± S.E.M. of the analysis of 7 independent experiments of 5 groups of 3 glands per treatment. Different letters above the columns indicate significant differences among treatments (unpaired t-test, ***P<0.001). B) JHAMT transcript levels: CA-CC complexes were dissected from 4 days old females fed on water or 20% sucrose. Transcript levels were measured by qPCR and are expressed as copy number of mRNA JHAMT/10,000 copies of rpL32 mRNA. Bars represent the means ± S.E.M. of 10 replicates of groups of 10 CA each (unpaired t-test, *P<0.05).