Angiopoietin-Like 4 Confers Resistance to Hypoxia/Serum Deprivation-Induced Apoptosis through PI3K/Akt and ERK1/2 Signaling Pathways in Mesenchymal Stem Cells
Figure 3
ANGPTL4 protected MSCs against hypoxia/SD-induced apoptosis.
ANGPTL4 (1–1000 ng/mL) was pre-incubated with the cells for 1 hr in complete medium before exposure to hypoxia/SD conditions, and then was maintained in the incubation medium throughout the hypoxia/SD treatment period. (A) Representative image of flow cytometric dot plots analyses of apoptotic cells after Annexin V/PI staining. (B) Image showing TUNEL+ cells in each group. (C and D) Composite values of apoptotic index and percentage of TUNEL+ cells. (Each column represents the mean ± SD of three independent experiments; *P<0.05 vs. hypoxia/SD). (E) Representative image of Western blots of procaspase-3 cleavage induced by hypoxia/SD and its inhibition by ANGPTL4 using anticaspase-3 antibody. (The blots shown are representative of three independent experiments). (F) Composite values of the ratio of cleaved caspase-3 and procaspase-3 relative to β-actin. (Each column represents the mean ± SD of three independent experiments; *P<0.05 vs. hypoxia/SD).