The Use of Anchored Agonists of Phagocytic Receptors for Cancer Immunotherapy: B16-F10 Murine Melanoma Model
Figure 6
Changes of intratumoral levels of IL-1beta during therapy based on the use of f-MLFKK-BAM, LPS and their mixture.
Groups of 9 mice received a single dose of 0.5-MLFKK-BAM, LPS (0.5 mg/ml), mixture of 0.5 mM f-MLFKK-BAM and LPS (0.5 mg/ml), and PBS alone in 50 μl i.t. 3 mice from each group were killed in 12, 24 and 48 hours intervals. After preparation of cells from excised tumors, corresponding supernatants were used for IL-1beta determination. IL-1beta levels are expressed as pg of IL-1beta/mm3 of tumor tissue.