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Quantitative Analysis of Microbicide Concentrations in Fluids, Gels and Tissues Using Confocal Raman Spectroscopy

Figure 9

Tenofovir concentrations (measured vs. computed) in the bottom fluid compartment in the Transwell assay.

A mass balance computation calculated the difference between the initial amount of Tenofovir in the fluid overlayer and the subsequent time-dependent sum of the total amounts of drug in the top layer and tissue. The good agreement between this mass balance prediction and the actual values measured supports the accuracy of the measurements. The box-and-whisker plot shows smallest value (lower bar), lower quartile (bottom of box), median (line through box), upper quartile (top of box), and largest observation (upper bar). The concentrations in the bottom fluid compartment from 0 to 9 h were not measurable (i.e., they were below the lower detection limit of the Raman quantification).

Figure 9

doi: https://doi.org/10.1371/journal.pone.0085124.g009