Activation of the Nrf2/HO-1 Antioxidant Pathway Contributes to the Protective Effects of Lycium Barbarum Polysaccharides in the Rodent Retina after Ischemia-Reperfusion-Induced Damage
Figure 4
LBP reduces ROS generation in the retina after ischemia-reperfusion.
ROS generation in fresh retinas was detected using DHE staining. (A) Representative micrographs of retinal sections stained with DHE (24 h after ischemia). (B) Quantitative analysis of ROS levels in the whole retina. The fluorescent intensities of the DHE-labeled neurons were quantified using an image analysis software program (Adobe Photoshop CS5; Adobe Corp.) (mean ± SEM, n = 5). ***p<0.001 compared to control, ### p<0.001 compared to I/R. Scale bar: 20 µm. Control: sham-operated animal, I/R: vehicle-treated animal with 1 h ischemia, and LBP+I/R: LBP-pretreated animal with 1 h ischemia. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer.