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Cloning and Characterization of a Novel Vacuolar Na+/H+ Antiporter Gene (Dgnhx1) from Chrysanthemum

Figure 5

Phenotype of DgNHX1-overexpression transgenic tobacco plants (OE-6, OE-9, and OE-13).

(A) The expression level of DgNHX1 in WT and DgNHX1-OE transgenic T2 lines. Ethidium bromide staining of PCR products using DgNHX1-specific primers with (top) and without (middle) prior reverse transcription, and RT-PCR products with NtUbiqutin-specific primers (bottom). (B) Phenotype of DgNHX1-overexpression transgenic tobacco plants.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0083702.g005