Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

A New In Vitro Model to Study Cellular Responses after Thermomechanical Damage in Monolayer Cultures

Figure 10

ROS synthesis at the area adjacent to thermally damaged HUVEC (tdH).

To detect ROS, the fluorochrome H2DCFDA was used: the intensity of the green fluorescent signal of the region limited by the inner ring was analysed. Representative images of ROS synthesis 30 min after thermal (A, A’) and after mechanical (B, B’) damage. A and B, green fluorescent ROS, merge of HUVEC (phase contrast) and green fluorescent ROS (‘). No ROS synthesis was detected after using the unheated stamp (B). Scale bar 500µm. C: quantification of the ROS synthesis normalised to the control. After 30 min, a 10-fold increase after thermal damage in HUVEC was observed compared to the unheated stamp control, and a 30-fold increase was observed after 1 h. Two hours after thermal damage the signal varied substantially, as is indicated by the error bar. The experiment was performed in triplicates, n>8 images were analysed.

Figure 10

doi: https://doi.org/10.1371/journal.pone.0082635.g010