Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

The Relationship between Membrane Potential and Calcium Dynamics in Glucose-Stimulated Beta Cell Syncytium in Acute Mouse Pancreas Tissue Slices

Figure 5

[Ca2+]i and membrane potential oscillations during stimulation with 12 mM glucose plus 10 mM tetraethylammonium (TEA).

A A high resolution image showing loading of cells with the calcium-sensitive dye OGB-1. B A color-coded representation of responsive cells from A with respect to time lags between the start50 of [Ca2+]i oscillation in the first cell and every other cell. C Time trace of a [Ca2+]i oscillation during stimulation with 12 mM glucose plus 10 mM TEA in the cell indicated in B. D A high resolution image showing labeling of cells with the voltage-sensitive dye VF. Note that almost exclusively plasma membranes are labeled. E A color-coded representation of responsive cells from D with respect to time lags between the start50 of membrane potential oscillation in the first cell and every other cell. F Time trace of a membrane potential oscillation during stimulation with 12 mM glucose plus 10 mM TEA in the cell indicated in D. The time scale is the same for C and F. Y axis represents the normalized fraction of the difference between maximum and plateau baseline fluorescence. G Durations of [Ca2+]i oscillations (OGB-1: 1st quartile=135 ms, median=166 ms, 3rd quartile=248 ms, n=57 cells from three islets) and membrane potential oscillations (VF: 1st quartile=81 ms, median=101 ms, 3rd quartile= 162 ms, n=107 cells from five islets) at their half-maximal amplitude. Asterisks indicate a statistically significant difference (p<0.001, Mann-Whitney rank sum test). H Velocities of [Ca2+]i waves (OGB-1: 1st quartile=372 µm/s, median=623 µm/s, 3rd quartile=1058 µm/s, n=8 values from three islets) and membrane potential waves (VF: 1st quartile=457 µm/s, median=577 µm/s, 3rd quartile=857 µm/s, n=12 values from five islets) were not statistically significantly different. Resolution was 256 x 64 pixels at 97 Hz for OGB-1 and 256x128 at 99Hz for VF.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0082374.g005