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3D-Printed Microwell Arrays for Ciona Microinjection and Timelapse Imaging

Figure 3

Ciona injection mold, prototype 2.

A) Top view of the design for the proposed device. B) Side view. C) Perspective view. D) Photograph of the device as built by micro-resolution stereolithography. E) Scanning electron micrograph of one subarray of posts on the 3D printed device. F) Confocal micrograph of micro-wells cast in agarose. The agarose is labelled with fluorescent beads. Orthogonal reconstructions through the confocal stack are as indicated. Scale bar=100µm.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0082307.g003