Structural Insights for Activation of Retinal Guanylate Cyclase by GCAP1
Figure 5
Structural characterization of GCAP1 mutants at the domain interface.
(A) Overlay of 15N-1H HSQC spectra of V77E (red), L82E (blue), WT (black) and W94F (green) indicate that each mutant is properly folded and structurally intact. The mutant spectra look similar to that of wildtype particularly for residues in structured regions. Minor spectral differences are observed for exposed residues in unstructured regions, most likely due to small differences in solvent conditions. (B) Expanded view of 15N-1H HSQC downfield region for GCAP1 mutants: V77E (red), L82E (blue), WT (black) and W94F (green). Three downfield NMR peaks assigned to G69, G105 and G149 indicate Ca2+ is bound functionally at EF2, EF3 and EF4 for each mutant.