Lack of RNase L Attenuates Macrophage Functions
Figure 4
RNase L regulates the expression of Cox-2.
RNase L deficient and wild type BMMs were treated with 1 µg/ml of LPS, 1,000 units/ml of IFN-α, 500 units/ml of IFN-γ, and 100 ng/ml of M-CSF for 14 h. The expression of Cox-2 was determined by Western blot analysis using a monolocnal antibody against mouse Cox-2 (A). RNase L wild type and deficient MEFs were treated with 1 µg/ml of LPS for 14 h. Total RNAs were isolated by using the Trizol Reagent (Invitrogen, CA). The expression of Cox-2, TNF-α, IL-6 and IL-1β was determined by RT-PCR (B). The expression of Cox-2 was measured by real-time PCR (C), which was performed twice in triplicate; the fold change from one of the experiments is present as Mean ±SD, *p<0.05. The production of PGE2 in the media culturing the two types of BMMs treated with LPS as described above was analyzed by ELISA (D). Experiments were performed twice in triplicates. Data are presented as Mean ±SD, *p<0.05.