Drosophila Growth Cones Advance by Forward Translocation of the Neuronal Cytoskeletal Meshwork In Vivo
Figure 2
Growth cones advance by forward translocation of the C-domain and axonal framework in vitro.
(A) Phase and (B) fluorescent images over 1 h of MitoTracker labelled Drosophila neurons grown on poly-ornithine. Kymographs of the phase images (C) and fluorescent images (D) show the position of the growth cone and mitochondria over time. (E) Green arrows overlaid on the kymograph illustrate the movement of docked mitochondria and the blue arrows show the tracks of fast transported mitochondria. The corresponding images from a neuron grown on DECM are shown in panels (F-J). Time arrow = 30 min and scale bar is 10 µm for both the time-lapse images and kymographs. (K) Quantitative analysis of the velocity of docked mitochondria plotted against distance from the growth cone. Errors bars are 95% confidence intervals. The numbers at the base of the bars denote the number of mitochondria analyzed in each bin. The growth cone is defined as the first 5 µm of axon.