C. elegans Aging Is Modulated by Hydrogen Sulfide and the sulfhydrylase/cysteine Synthase cysl-2
Figure 1
The slow-releasing H2S donor GYY4137 mediates an aging and stress response in C. elegans.
(A)Intracellular levels of ROS were obtained from age-synchronized worms assayed at L4 stage following treatment with vehicle (control) or GYY4137 (100 µM) for 48 hours in liquid medium. Each bar represents data from 3 independent experiments with at least 2000 worms in each treatment group and normalized by the calculated protein content (* and ***, statistically significant c.f. control; p < 0.05 and p < 0.001, respectively). GYY4137 treatment improved (B) survival and (C-D) growth (body area and length) in wild type worms. Nematodes were exposed to the drug from L1 larval stage until death (day of death refers to the age (days) post L1); survival curves were plotted by the Kaplan-Meier method and median survival values were compared by log-rank (Mantel-Cox) test with 95% significance using GraphPad Prism v5 software. GYY4137 treatment improves the resistance of wild type nematodes to (E) oxidative, (F) ER and (H) thermal stressors, but notably not to (G) cadmium-induced stress.