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A Simple Alkaline Method for Decellularizing Human Amniotic Membrane for Cell Culture

Figure 7

Phase contrast of various cells grown on NaOH-denuded HAM.

Note cobblestone monolayer of telomerase-immortalized corneal epithelial cells (tHCEC), LESC, and LESC-derived iPSC. Fibroblast-derived iPSC also show good spreading of cells and growth. Bar = 40 µm.

Figure 7

doi: https://doi.org/10.1371/journal.pone.0079632.g007