Frizzled-5 Receptor Is Involved in Neuronal Polarity and Morphogenesis of Hippocampal Neurons
Figure 3
Fzd5 overexpression alters the polarity of hippocampal neurons.
A. Immunodetection of the axonal markers MAP1B-P and Tau1 in neurons 24 h after transfection with the empty vector PCS2+/GFP as a control or Fzd5-HA/GFP at 2 DIV. GFP was used to identify transfected neurons. Representative images, shown in panels a and b, of control neurons expressing the axonal markers distributed in one neurite and in panels c and d, neurons that overexpress Fzd5 with the axonal marker distributed in the whole cell. B. Quantification of neurons transfected on 1-3 DIV with polarized distribution of an axonal marker. The percentage of neurons with polarized axonal markers was significantly lower in neurons overexpressing Fzd5 than in control neurons, in a DIV-dependent manner. Scale bar: 20 µm. Bars represent mean ±SEM of three independent experiments. *** p< 0.001.