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High-Level Expression of Bacillus naganoensis Pullulanase from Recombinant Escherichia coli with Auto-Induction: Effect of lac Operator

Figure 2

SDS-PAGE analysis of PUL expression in E. coli BL21(DE3)/pET-20b(+)-pul before and after degeneration.

Lane M: protein molecular weight marker; Lane 1: total protein after degeneration; Lane 2: total protein before degeneration.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0078416.g002