Slow Proton Transfer Coupled to Unfolding Explains the Puzzling Results of Single-Molecule Experiments on BBL, a Paradigmatic Downhill Folding Protein
Figure 5
pH dependence of the two phases observed in the T-jump experiments of BBL at 279 k near the chemical denaturation midpoint.
The fast phase is shown in red and the slow phase in blue. The error bars signify the fitting errors to the double exponential fits of Fig. 4. The blue and red curves are the predictions by the 1D free energy surface model (see Fig. 6).