A Neuron-Based Screening Platform for Optimizing Genetically-Encoded Calcium Indicators
Figure 6
Neuronal culture platform performance parameters and detection sensitivity.
(A) GCaMP3 basal fluorescence (F0) relationship with mCherry fluorescence (249 wells). (B) ∆F/F0 (10 AP) response relationship with mCherry fluorescence. (C) mCherry fluorescence relationship with number of infected cells. (D) Median ∆F/F0 (10 AP) response dependency on number of infected cells. (E) Percent detectable improvement relative to GCaMP3 performance was estimated by simulating 105 experiments using 3 to 12 replicate wells drawn from a data set of 249 GCaMP3 wells. The difference between the mean and the 99th percentile of simulated result distributions normalized by the mean defined the detection sensitivity at α=0.01 (red: 1 AP, green: 3 AP, blue: 10 AP, magenta: 160 AP, black: decay time (10 AP)). (F) After compensation correcting for infected cell density effect.