Expansion of the Gateway MultiSite Recombination Cloning Toolkit
Figure 4
Functional demonstration of the L1-LexAp65-R5 Gateway MultiSite entry clone.
Representative confocal images of third instar larval expression of (A) yw; iav-LexAp65/LexAop-mCD8GFP; (B-C) yw; n-syb-LexAp65/+; LexAop-mCD8GFP/+. iav-LexAp65 expresses exclusively in chordotonal organs. n-syb-LexAp65 expresses broadly in the CNS (B) and sensory and motor neurons (C). Anti-GFP was used as the primary antibody for (A) and (C). Direct GFP fluorescence was used for imaging (B). Scale bars: (A) 200μm, (B) 100μm, (C) 200μm.