BMP-2 Induces Versican and Hyaluronan That Contribute to Post-EMT AV Cushion Cell Migration
Figure 7
Quantitative evaluation of cell migration by HH stage-24 AV CMC aggregate cultures treated with HA oligomers.
(A) CMC aggregate cultures were treated with HA oligomers (100 µg/ml) or chitin oligomers (control oligomers) (100 µg/ml). HA oligomer treatment significantly reduced cell migration, whereas control chitin oligomer treatment did not significantly alter cell migration. *p<0.01; **p<0.001 as compared to the untreated control (M199). (B) BMP-2 (200 ng/ml) promoted cell migration. BMP-2-promoted cell migration was significantly reduced by adding HA oligomers (100 µg/ml) to the CMC aggregate cultures, whereas chitin oligomer treatment did not significantly reduce BMP-2-promoted cell migration. The asterisks on the green curve show significant differences between BMP-2 treatment and the untreated control (M199) (blue curve). The asterisks on the purple curve indicate significant differences between treatment with BMP-2+ HA oligomers and treatment with BMP-2 (green curve). Vertical bars indicate ± SD of the mean. *p<0.01; **p<0.001. A and B represent quantitation of results for each culture condition from a minimum of three experiments, in each of which three separate aggregates were examined.