The Impact of KLF2 Modulation on the Transcriptional Program and Function of CD8 T Cells
Figure 5
KLF2 re-expression inhibits proliferation but not via c-myc repression.
(A) Data show the cellular DNA content of GFP-KLF2neg and GFP-KLF2pos activated CD8 T cells. Data are representative of 4 independent experiments. (B) Expression of c-myc mRNA in FACS purified activated CD8 T cells quantified by qRT-PCR (data normalised to GFPneg and show mean + SEM of 3 independent experiments). (C) DNA synthesis measured by EdU uptake in the T cell populations shown (data show mean percentage EdU uptake + SEM, n=3). (D) Cell counts of GFPpos and KLF2pos activated CD8 T cells; data shown are mean ± SEM of 3 independent experiments.