Direct Induction of Chondrogenic Cells from Human Dermal Fibroblast Culture by Defined Factors
Figure 4
Characterization of matrix of pellet culture of iChon cells.
The iChon cells at passage 7 were used. (A) After 3 weeks of culture, pellets of iChon cells (# 117-3), chondrogenically differentiated human bone bone marrow stem cells (CD-hBMSC), and human dermal fibroblasts (HDFs) were recovered and processed for histological sections. Semiserial sections were stained with toluidine blue and picrosirius red. Sections stained with picrosirius red were observed under polarized microscopy. Bars in the top and third rows, 500 µm; Bars in the second and bottom rows, 50 µm. (B) Semiserial sections of pellets of iChon cells (#117-37) after 3weeks of culture were stained with toluidine blue, and immunostained with anti-type I collagen antibodies and anti-type II collagen antibodies. Bars, 100 µm. (C) Control for immunohistological analysis in (B). Histological sections from osteochondroma samples dissected at a time of surgery were immunostained with anti-type I collagen and anti-type II collagen antibodies. Panels are magnification of boxed regions in Figure S7. Bar, 100 µm. C, cartilage; B, bone.