Neuroglobin Over Expressing Mice: Expression Pattern and Effect on Brain Ischemic Infarct Size
Figure 9
Ngb-IR in the penumbra and infarct volumes 24 hours after permanent middle cerebral artery occlusion.
Ngb-IR in the cortex of a representative pMCAo operated Ngb-Tg mouse is shown in A. No increase in Ngb-IR intensity was observed in pMCAo operated Ngb-Tg mice when compared to sham-operated Ngb-Tg mice (B). The red line marks the penumbra. Black arrows exemplify Ngb-IR neurons and a black arrowhead a necrotic cell. No increase in Ngb expression is seen within nor adjacent to the penumbra area in the pMCAo operated Ngb-Tg animals compared to Tg-sham (A-B), which suggests no selective sparing of neurons expressing Ngb. Infarct volumes 24 hours after pMCAo estimated with 2D nucleator and Cavalierís principle is shown in C. The estimated infarct volume in cortex was significantly larger in WT Tg background mice (n = 5, total mean volume 3.5 mm3 SE±0.77 mm3) compared to Ngb-Tg (n = 7, total mean volume 1.7 mm3 SE±0.27 mm3) littermates. p<0.048. Also infarct volumes between the two different WT background strains differed significantly. The infarct volume of the WT mice with hsa1 background (n = 4 total mean volume 6.2 mm3 SE±0.26 mm3), was significantly larger compared to the infarct volume in the WT mice with Tg-background, p<0.032. Mean values are indicated by the lines and * denotes significance. Scale bar 50 µm.