Therapeutic Potential of the Translation Inhibitor Silvestrol in Hepatocellular Cancer
Figure 7
Estimation of tumor growth by bioluminescence.
A, In vitro bioluminescence imaging of PLC-luc cells. Cells were counted and seeded on black 96-well plates. D-luciferin (150 µg/ml) was added to each well and imaging was performed using IVIS. Bioluminescence is plotted against cell number. B, In vivo bioluminescence imaging. Orthotopic tumors were established by direct intra-hepatic injection of PLC-luc cells. One million PLC-luc cells were injected into the left lobe of the liver. The growth of tumors were monitored by bioluminescent imaging using the IVIS. C, The relation between the tumor volume and bioluminescence. After at least 4 weeks of monitoring mice were sacrificed and liver tumor volumes were obtained using caliper measurement. The estimated volume of tumor after excision is plotted against bioluminescence determined in situ.