The Voltage-Gated Sodium Channel Nav1.8 Is Expressed in Human Sperm
Figure 4
Effects of veratridine on human sperm motility in the presence of tetrodotoxin, A-803467 or ab-66743.
The effects of veratridine (10 μM) after different incubation times were analyzed in the presence of (A) the VGSC inhibitor tetrodotoxin (TTX) (10 nM) (B) TTX (10 μM), (C) the selective Na v1.8 antagonist A-803467 (10 μM), (D) the Na v1.8 antibody ab-66743 (dilution 1:50) or the corresponding solvent. Bars are means with SEM of 6-8 different experiments and represent percentage changes in progressive motility (grade A+B sperm) relative to the value observed at the same time in the respective solvent-treated paired controls. *P<0.05, significant difference vs. responses to veratridine in the presence of the antagonist or antibody solvent.