AAV-Mediated, Optogenetic Ablation of Müller Glia Leads to Structural and Functional Changes in the Mouse Retina
Figure 5
Structural changes following Müller cell ablation.
A) OCT imaging collected over the course of 8 weeks reveals the progression of structural changes following a single 2 hour irradiation. Blurring of the IS/OS was visible 1.5 days after irradiation (red arrows). Disorganization of the ONL was visible 2 weeks after ablation (yellow arrows). Inner retinal changes were apparent 8 weeks after irradiation (blue arrows). B) Quantification of thickness of retinal layers from OCT images performed 5 months after irradiation (n=5) revealed that the overall thickness of the retina was only slightly (but not significantly) decreased. In contrast, a decrease in the thickness of photoreceptor inner and outer segments was significant, as was a decrease in the thickness of the ONL (*=P<0.05).