Identification of Mur34 as the Novel Negative Regulator Responsible for the Biosynthesis of Muraymycin in Streptomyces sp. NRRL30471
Figure 4
(A) SDS-PAGE analysis of His6Mur34, the theoretical molecular mass of His6Mur34 is 19.6 kDa. The Mur34 protein was loaded into 12% SDS-PAGE for analysis. (B) EMSA analysis of Mur34 and mur33 promoter. For the above figure, 50-fold of poly dI-dC was added to the each reaction system with an increasing amount of Mur34. For the competitive assay (below), lower case of all samples contain 2.6×10−4 M promoter DNA of mur33 (90-bp specific DNA), for samples 2, 3 and 4, extra 9×10−6 M His6Mur34 was individually contained. Moreover, 50-fold of unlabelled competitive DNA was added to the reaction system (band 3), and 50-fold of unspecific non-competitive DNA to the system (band 4). Band designations, 1, free DNA; 2-4, protein-DNA complexes.