The Housekeeping Gene Hypoxanthine Guanine Phosphoribosyltransferase (HPRT) Regulates Multiple Developmental and Metabolic Pathways of Murine Embryonic Stem Cell Neuronal Differentiation
Figure 4
Immunohistochemical detection of the neuronal marker β-III tubulin and tyrosine hydroxylase (TH) in ESD3 cells at the final 14-day neuronal stage of SNM differentiation.
(A) β-III tubulin in WT control cells; (B) TH expression in control WT cells; (C) merged β-III tubulin and TH expression in WT control cells; (D) β-III tubulin in HPRT-knockdown cells; (E) TH in HPRT-knockdown cells, and (F) merged β-III tubulin and TH expression in HPRT-knockdown cells.