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Genome Analysis Coupled with Physiological Studies Reveals a Diverse Nitrogen Metabolism in Methylocystis sp. Strain SC2

Figure 8

Functional classification of genes identified in members of the Methylocystaceae.

The gene content of strain SC2 (red), strain Rockwell (blue) and Ms. trichosporium OB3b (green) and that of the core genome shared by them (grey) was subjected to functional classification by the RAST server. CDS were classified into 27 functional categories using the SEED subsystem. Numbers in parentheses next to the strain names indicate the number of CDS assigned to the SEED subsystem out of the total number of CDS present in the particular genome. The proportion of CDS (x-axis) assigned to a particular subsystem was calculated by dividing the number of CDS assigned to this category by the total number of CDS assigned to the SEED subsystem database. The functional categories were arranged according to the number of CDS assigned for strain SC2 to each category. The number of CDS classified for the individual strains and their core genome into each SEED subsystem was subjected to statistical analysis using STAMP. A p-value cutoff of 0.05 was used to determine significant differences. Subsystems showing significant differences are marked by an asterisk.

Figure 8

doi: https://doi.org/10.1371/journal.pone.0074767.g008