Two Wheat Glutathione Peroxidase Genes Whose Products Are Located in Chloroplasts Improve Salt and H2O2 Tolerances in Arabidopsis
Figure 4
GPX activity analysis in pET-28a, pET-W69 and pET-W106.
(A) SDS-PAGE analysis of the expression of recombinant proteins in E. coli. The complete protein of the bacteria and purified recombinant protein from soluble crude extract were separated by 12% SDS-PAGE and stained with Coomassie Brilliant Blue. M, protein size marker (16–94 kDa). T, total proteins of the bacteria (10 μg); P, purified recombinant proteins (1 μg) from soluble crude extract. (B) Enzyme activities of the GPX isoenzymes were calculated with H2O2 and t-BHP as the substrates. Data are means ±SD of three independent experiments.