SOX2 and OCT4 mRNA-Expressing Cells, Detected by Molecular Beacons, Localize to the Center of Neurospheres during Differentiation
Figure 4
Tracking OCT4-positive cells inside neurospheres during differentiation.
A. 24h after initiation of differentiation, OCT4-positive cells are still diffusely spread in some neurospheres, while in others they start localizing to the inner core. B. 48h later the neurospheres attach to the matrix and the cells located around the rim start to form neurites. Neurospheres demonstrate varying sizes and differentiation potential. C. Fusion between neurospheres was seen, together with neurite formation D. In both cases, the MB-detected OCT4-positive cell population was located in the middle of neurospheres. Images were acquired with ApoTome Imaging system, 10x/0.3 Plan Neofluar objective and DsRed filter set.