Unique Anti-Glioblastoma Activities of Hypericin Are at the Crossroad of Biochemical and Epigenetic Events and Culminate in Tumor Cell Differentiation
Figure 2
Induction of GBM tumor cell differentiation following exposure of these GBM cell lines to HYP (20 µM).
(A) Changes in cell morphology; staining with Hemacolor reagents. (B) Expression of neuro-glial differentiation antigens GFAP (glial), βIII-tubulin (neuronal) and NeuN (early neuronal nuclear) in HYP-treated U87-MG cells, (C) T98G cells, (D) U251-MG cells. Immunocytochemical staining. Right exhibit in each line reflects samples with nuclear counterstaining with DAPI.