Normal CFTR Inhibits Epidermal Growth Factor Receptor-Dependent Pro-Inflammatory Chemokine Production in Human Airway Epithelial Cells
Figure 7
IL-1alpha binding to IL-1R increases EGFR-P and IL-8 production in IB3 cells but not in C38 cells.
A, B. EGFR-P (A) or IL-8 (B) was measured in C38 cells (open bars) and in IB3 cells (hatched bars) incubated for 1 h (A) or 24 h (B) with medium alone (–; control). Neutralizing antibodies were not added (–) or neutralizing antibodies against IL-1R (10 mcg/ml), IL-1alpha (5 mcg/ml), or IL-1beta (5 mcg/ml) were added at time 0 h. C. IL-1alpha was measured in cell supernatants of C38 cells (open bar) and IB3 cells (hatched bar) incubated for 1 h with medium alone. A-C. Values are means ± SD; n = 5. P<0.05 compared with control (*; A, B) or compared with the C38 cells (*; C).