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Organelle and Cellular Abnormalities Associated with Hippocampal Heterotopia in Neonatal Doublecortin Knockout Mice

Figure 7

Apoptosis is increased in the postnatal Dcx KO hippocampus.

Immunoreactivity to activated caspase-3, and Hoechst staining observed in P2 WT (A, C, E) and Dcx KO (B, D, F) dorsal hippocampi at rostral (A, B) and more caudal (C, D) hippocampal levels. Arrowheads in A–D indicate the positions of labelled cells, which had typical features for apoptotic cells. E and F are higher magnifications corresponding to the inset regions in C and D. Caspase-3 positive cells are present in both the stratum oriens (SO) and some in the stratum pyramidale (SP) of the CA3 region of both WT (E) and KO (F) hippocampi. (G, H) Histograms represent the cellular density of activated caspase-3 expressing cells in CA1 and CA3 regions respectively of WT versus KO hippocampi. Note the increase in activated caspase 3-positive cells in the Dcx KO, p<0.05. Scale bars: A–D: 100 µm; E–F: 50 µm.

Figure 7

doi: https://doi.org/10.1371/journal.pone.0072622.g007