Human Glioblastoma Multiforme: p53 Reactivation by a Novel MDM2 Inhibitor
Figure 16
Tumors were harvested from mice of different ISA27 treatment groups (IP = intraperitoneal treatment; IT = intratumor treatment), processed to obtain cell lysates for Western blot and total RNA for real-time RT-PCR analysis. A) Western blot analysis showed up-regulation of p53 and p53 transcriptional target p21 in tumors from ISA27 IT group and up-regulation of p53 in tumors from ISA27 IP group. In this group, the increase of p21 was near to significance. B) Real-time RT-PCR analysis showed a statistically significant increase in MDM2, p21 and PUMA mRNA levels in tumors from ISA27 IT and IP groups.